🧬 For our science-loving readers
Guest post by Petra Davelaar, ND, CNS, IFMCP
Genetic consequences of COVID-19 mRNA vaccines and how deuterium depletion can be one of the most important ways to mitigate disease.
Pathological processes triggered by this injected synthetic pathogen-stimulating-computer-generated sequence of mRNA, marketed – and increasingly being mandated –– on a global level, include acute, immediate, and those that can appear in a matter of days, weeks, months and, if ‘lucky’, years after the introduction of these gene therapies.
Immediate Genetic Changes
To describe in detail the significance of the immediate genetic pathological processes that are triggered upon the introduction of the synthetic pathogen-stimulating-computer-generated sequence of mRNA –– which starts synthesizing genetically engineered viral spike proteins immediately upon translation–– it is imperative to understand the fundamentals of the function and lifespan of mRNA.
RNA is the abbreviation for ribonucleic acid. Coding RNA provides “working copies” of the genetic master plan of an organism that is contained in the sequence of deoxyribonucleotides in its deoxyribonucleic acid (DNA). Transcription is the copying process during which a DNA strand serves as a template for the synthesis of RNA. Transcription produces messenger RNAs that are then translated into sequences of amino acids or proteins, as well as various other non-coding RNAs that are not translated but perform catalytic, regulatory, and structural functions. Up until very recently, it was believed that only an exogenous reverse transcriptase, as present in retroviruses such as HIV, could change the direction of this copying process.
Central Dogma of Biology DNA→RNA→Protein Does No Longer Hold
On June 11, 2021 Science Advances, one of the Open Access periodicals of the American Association for the Advancement of Science (AAAS) published a landmark paper¹ that challenges the central dogma of biology namely the understanding that DNA is transcribed into RNA and then RNA is translated into protein, irreversibly. It turns out that one of the 14 human DNA polymerases that have been identified in humans, called Polymerase Theta (Polθ), known as an error-prone and promiscuous enzyme, is more efficient than HIV reverse transcriptase in converting mRNA sequences or fragments into DNA when stable mRNA templates are available. This process is regulated by what is called substrate level regulation, meaning, this reaction will only take place if there is stable mRNA available, i.e. the substrate of the reaction.
mRNAs under normal physiological conditions break down in seconds to minutes after translation in cells, tissues and/or the circulation. This has been a long-standing technically challenging problem in RNA vaccine research. The rapid and spontaneous breakdown of RNA occurs to preserve genetic integrity of the body by preventing the reverse transcriptase action of any polymerase, especially that of the theta isoform. Additional mechanisms that quickly degrade and/or modify RNA molecules include nucleases, involved in cleaving the phosphodiester bonds between nucleotides².
Evading Detection and Rapid Break Down of mRNA
To prevent this rapid breakdown of mRNA, vaccine developers replaced one of the four letters that makes up the alphabet of RNA (ACGU). They replaced the U, which stands for uracil with a Y for pseudo-uridylyl, which allows it to evade receptors that can detect the nucleoside-modified messenger RNA (modRNA)³ and destroy it⁴. Geneticists⁵ are rightfully alarmed by this experimental therapy because never before has the body encountered entire sequences of RNA made with pseudo-uridylyl. The body knows fragments of pseudo-uridylyl but the BioNTech/Pfizer vaccine contains a sequence that is 4,284 characters long. RNA nucleases do not recognize these synthetic structures and modified mRNA sequences will persist. A single dose of the Moderna vaccine reportedly contains 40 trillion mRNA molecules.
Stabilization of the Synthetic Modified mRNA and the Nano Lipidparticles with Deuterium
In order to stabilize the modified mRNA sequence itself, vaccine developers produce them in heavy water. Deuterium is the stable isotope of hydrogen that appears naturally in all water on Earth. If we change the ratio of the deuterium to hydrogen content (increasing deuterium), we create “heavy water”, best known for slowing down atomic destabilization reactions in nuclear facilities. This is because of the double weight and size that cause significant kinetic isotope effects of deuterium. How does this relate to stabilization of mRNA? Because the presence of deuterium increases the binding strengths and affinities as RNA undergoes a conformational change (its shape is different and therefore the folding of the structure is altered). Deuterium can either be present in the vaccine (as the solvent or in mRNA) itself or in the possibly deuterium-loaded cytoplasm⁶ of the cell of the host. These processes stabilize mRNA so that it will break down much, much slower. This is not new information. Chemically modified mRNAs for use in vaccines have been made with heavy water (Deuterium-rich water) for stability⁷ since at least 2009. Additionally, the nano lipid particles (NLP) are also deuterated to make them persist⁸. Watch the promotional videos of the manufacturer here⁹.
Fidelity of Polymerase Theta
Polymerase theta is present in both the nucleus and the cytosol. It is a DNA polymerase, meaning its function is to repair double-strand breaks in DNA, except when there is RNA around. Polymerase theta has a preference for binding RNA as its template while using deoxynuleotides for its polymerized product. A unique property of the reverse transcriptase activity of polymerase theta, seen in all reverse transcriptase enzymes, is that it transfers two protons in the process of pairing and transcribing RNA nucleotides into DNA. These protons come from water¹⁰. If the water in the cytoplasm is deuterated, meaning it has an increased ratio of deuterium to hydrogen, the likelihood the polymerase theta picks up a deuterium instead of hydrogen is increased as well. The addition of a deuterium atom into the newly formed DNA increases the size and hydrogen (1H; P+) bond strength in DNA by about a magnitude as deuterium’s nucleus (2H; P+N) is twice the size and weight of hydrogen. If this happens more frequently, the cell will end up with additional DNA in the cytoplasm or nucleus or both. A normal human cell wraps all newly synthesized DNA in 46 chromosomes before dividing. If there is too much DNA, the cell will produce additional chromosomes to wrap it all up neatly. This is called aneuploidy or cancer. For example, a pancreatic cancer cell (MIA PaCa) will have 52 chromosomes, a metastatic lung cancer cell 61 (H441). This is due to excess deuterium present in the cell and the speed of the reverse transcription of RNA into DNA depends on the presence of stable “immortalized” mRNA. What do you suppose happens when the synthetic modified mRNA has been introduced into the body and is taken up into the cell after the nano-lipid membranes fuse with the cellular membrane and it enters the cytoplasm?
How Do Cells Become Loaded with Deuterium?
Consuming a diet of foods and liquids high in deuterium content will cause the body to accumulate excess deuterium. If that is combined with modern living, poor mitochondrial functions, low oxygen saturation, you will not be able to excrete the overload of deuterium. Deuterium is an oncoistope exactly because of the process described above. Deuterium depletion is a foundational intervention that is best adopted by all. The above mechanisms are now published in Science Advances as an eLetter by my team of collaborators¹¹.
“We Now Have Unambiguous Evidence¹²”
Although the exact sequences that are present in the vaccines seem to be elusive, what has been shown is that these gene therapies facilitate permanent genetic expression via endogenous reverse transcriptase polymerase theta. A recent PNAS paper confirms the integration of the SARS-CoV-2 mRNA into the human genome¹³.
1 Chandramouly G, Zhao J, McDevitt S, Rusanov T, Hoang T, Borisonnik N, Treddinick T, Lopezcolorado FW, Kent T, Siddique LA, Mallon J, Huhn J, Shoda Z, Kashkina E, Brambati A, Stark JM, Chen XS, Pomerantz RT. Polθ reverse transcribes RNA and promotes RNA-templated DNA repair. Sci Adv. 2021 Jun 11;7(24):eabf1771. doi: 10.1126/sciadv.abf1771. PMID: 34117057; PMCID: PMC8195485.
7 Arnab Sen, Vinayagamurthy Balamurugan, Kaushal Kishor Rajak, Soumendu Chakravarti, Veerakyathappa Bhanuprakash & Raj Kumar Singh (2009) Role of heavy water in biological sciences with an emphasis on thermostabilization of vaccines, Expert Review of Vaccines, 8:11, 1587-1602, DOI: 10.1586/erv.09.105
10 C. Castro, E. Smidansky, K. R. Maksimchuk, J. J. Arnold, V. S. Korneeva, M. Götte, W. Konigsberg, C. E. Cameron. Two proton transfers in the transition state for nucleotidyl transfer catalyzed by RNA- and DNA-dependent RNA and DNA polymerases. Proc. Natl. Acad. Sci. U S A. (2007) 104, 4267-72. doi: 10.1073/pnas.0608952104
11 Boros, LG., et al. To stabilize or not to stabilize RNA – that is still the question. eLetters Re: Chandramouly, G., et al. Polθ reverse transcribes RNA and promotes RNA-templated DNA repair. Sci Adv. 2021 Jun 11; 7(24): eabf1771. https://advances.sciencemag.org/content/7/24/eabf1771/tab-e-letters
13 Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissuesLiguo Zhang, Alexsia Richards, M. Inmaculada Barrasa, Stephen H. Hughes, Richard A. Young, Rudolf Jaenisch Proceedings of the National Academy of Sciences May 2021, 118 (21) e2105968118; DOI: 10.1073/pnas.2105968118